ELISA or enzyme linked immunosorbent assay, is a plate-based technique that can detect and quantify soluble substances, such as peptides and proteins. The same technology can also be called enzyme immunoassay (EIA) or other names such as enzyme-linked immunosorbent assay (ELISA).
An ELISA uses the target macromolecule (antigen) to immobilize on a microplate and then complex it with an antibody that is linked with a reporter enzyme. The reporter enzyme's activity is measured by incubating with the substrate. This produces a measurable product. ELISA equipment requires a specific antibody-antigen interaction to be effective.
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ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. Antigens are harmful substances that the body's immune system detects and produces antibodies. It is necessary to take a blood sample. Most of the time, blood is drawn from a vein located on the inside of the elbow or the back of the hand.
ELISA kits can be used to quickly and accurately identify and quantify targets in samples and cultures. There are many options available, including competitive ELISA and sandwich ELISA, as well cell-based ELISA Kits that can be used to identify many targets. These ELISA kits come with pre-coated plates that are coated with standards, buffers, capture/detection antibodies and other reagents.
To ensure precision, accuracy and reproducibility, each kit is subject to fit-for-purpose validation. The enzyme-linked immunosorbent assay is a powerful tool for detecting and quantifying a particular protein in a complex mixture. ELISAs are usually performed on 96-well and 384-well polystyrene plate, which passively bind proteins and antibodies.